Localization of the Major Ethidium Bromide Binding Site on tRNA

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Localization of the major ethidium bromide binding site on tRNA.

Binding of ethidium bromide to Escherichia coli tRNAVal and an RNA minihelix based on the acceptor stem and T-arm of tRNAVal was investigated by 19F and 1H NMR spectroscopy of RNAs labeled with fluorine by incorporation of 5-fluorouracil. Ethidium bromide selectively intercalates into the acceptor stem of the tRNAVal. More than one ethidium bromide binding site is found in the acceptor stem, th...

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 1997

ISSN: 0305-1048,1362-4962

DOI: 10.1093/nar/25.19.3944